Teaching serial dilution




















Serial dilution allows estimation of a range of bacterial population sizes. Percent resistance can be calculated by culturing bacteria on both antibiotic-free and antibiotic-containing media and comparing the results.

In this Spreadsheet Across the Curriculum module, students explore the quantitative concepts of ratios and percentages and then apply those concepts to build Excel sheets that calculate bacterial population size and percent resistance. Quantitive Goals: Students will develop a deeper and more explicit understanding of proportions, ratios, and populations of inference.

They can also review scientific notation if necessary. Spreadsheet Goals: Students will learn to use simple mathematical formulas in Excel. Prices Free.

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All Resource Types. Results for serial dilutions 90 results. Sort: Relevance. Word Document File. This teaches students about powers of ten, concentration of pollution, and the concept of atoms. Show more details. Wish List.

Serial Dilutions Homework. Understanding how to perform serial dilutions are mandatory skills required of any chemistry student. ChemistryNinja puts together a homework assignment that allows students to learn how to perform dilutions, which they will then transfer that information to a dilution table.

This will prepare th. Biology , Chemistry , General Science. Homework , Laboratory , Worksheets. These instructions are supplemental to The Effect of Cell size and Osmolarity on Diffusion Rates and is also available as part of a bundle. As my students will be conducting independent investigations during the year, I like for them to learn to do many of the tasks generally assigned to the teacher.

Biology , International Baccalaureate. Handouts , Laboratory. Serial Dilutions Lab. This lab is an updated version of a lab often done in HS Chemistry classes. This one focuses on environmental contaminants such as PFOA or lead. Biology , Environment , Science. Activities , Laboratory , Projects.

Serial Dilution. Learners will be able to. Identify the concentration of solutions. Describe how to calculate concentration. Create a method for serial dilutions. Chemistry , General Science. Activities , PowerPoint Presentations. Serial Dilutions. This worksheet defines stock solution, serial dilution and provides 3 practice problems. All materials were written for and are currently used. Chemistry , Science. Students will observe and test the affects of a serial dilution on the pH of common household products.

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Method 1. Determine the proper dilution liquid. The liquid that you will be diluting your substance in is very important. Many solutions will be diluted in distilled water, but this is not always the case.

If you are diluting bacteria or other cells, you will likely want to dilute in culture media. Prepare several test tubes with 9 mL of dilution liquid. These tubes will serve as your dilution blanks. Each tube will be a fold dilution starting from the undiluted tube.

Prepare a test tube with at least 2 mL of your undiluted solution. The minimum amount needed to perform this serial dilution is 1 mL of undiluted solution. If you only have 1 mL you will not have any remaining undiluted solution. Label this tube US for undiluted solution. Thoroughly mix your solution before starting any dilutions. Perform the first dilution. Draw 1 mL of undiluted solution from test tube US with a pipette and transfer it to the test tube labeled containing 9 mL of the dilution liquid and mix thoroughly.

There is now 1mL of the undiluted solution in 9 mL of the dilution liquid. The solution, therefore, has been diluted by a factor of Perform the second dilution. For the second serial dilution, you will take 1 mL of solution from tube and add it to the 9 mL of dilution liquid in the tube Thoroughly mix tube before adding to the next tube. Again, mix the tube following dilution.

The solution from test tube has been diluted fold into test tube Extend this procedure to perform longer serial dilutions. This process may be repeated as many times as necessary to achieve the desired solution. In an experiment involving concentration curves, you can use a serial dilution to create a series of solutions with dilutions of 1, , , , Method 2.



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